Friday, September 30

LC Mass Spectrometry: Definition & Importance

LC-MS/MS is widely used and offers outstanding sensitivity. Triple-quadrupole mass spectrometry (MS/MS) offers additional benefits due to its selectivity. It can only distinguish compounds by their mass-to-charge ratios (m/z), which is inadequate for most real-world applications. Liquid chromatography mass spectrometry (LC-MS) is a powerful technique widely used in many laboratories. It has shown to be a powerful, qualitative and quantitative analytical tool. LC/MS offers outstanding sensitivity. Triple-quadrupole mass spectrometry MS/MS offers additional benefits due to its selectivity, which makes it an important constituent component of modern research and clinical laboratories worldwide. 

In most applications, it is essential to isolate compounds from samples containing thousands of other diverse molecules. This can only be done using multi-step analyses by LC-MS/MS or lc mass spectrometry, which are time-consuming and laborious. Graphical analysis software (GAS/LC) automates these processes and is widely used in various laboratories. Users can analyze multiple conditions at once with a single software, cutting down on workloads. As applied to environmental chemistry, GAS/LC offers benefits over traditional chromatographic techniques due to its multiple detection modes and powerful search capabilities that utilize non-targeted data within the chromatography column (advantageously avoiding potential interferences).

LC-MS/MS is a unique and powerful analytical technique that can be used with different configurations. It uses the same chromatography column for separation of analyte and other compounds, but different kinds of ionization techniques. In other words, it allows to separate analytes from various unknowns by making use of different kinds of LC column housings (and its polarity). 

PK PD analysis study links drug exposure to therapeutic effect measures so drug developers can better understand the relationships between exposure, efficacy, and toxicity. Thus, PK PD assay and data analysis results are essential to any ECTD submission. PK PD analysis studies links drug exposure to therapeutic effect measures so drug developers can better understand the relationships between exposure, efficacy, and toxicity. Thus, PK PD assay and data analysis results are essential to any ECTD submission. Non-compartmental analysis is a widely used PK method to quantify test article exposure. Detailed description of this approach can be found in pharmokinetics textbooks, such as Goodman et al.,1994; Ward et al., 2004). However, for our purpose the Kundera and Goodwin (2005) model will be used for the illustration studies. The Kundera and Goodwin model is based on two predicted regions A and B of the TOF/PK curve.  

PK PD assay and data analysis results are essential to any ECTD submission. Pharmacokinetics (PK) parameters are typically calculated by non compartmental analysis (NCA) following the determination of test article concentrations in samples from clinical or preclinical studies. Generally, area under the curve (AUC) and maximum concentration (Cmax, or C0 for an IV dose) are accepted as the most critical PK parameters when discussing exposure and activity or toxicity. AUC in pharmacokinetics represents the total exposure of a drug over time to a test subject or animal receiving this drug pharmaceutical agent. AUC can be extrapolated to infinity for well-designed studies

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